10.06.2020

QUESTION 3
During the isolation of Bacterial DNA, the mixture (Cell debris, genetic material, and proteins in buffers) is transfered to a centrifuge tube and treated with an equal volume of chloroform:isoamyl alcohol (24:1 v/v) solution. This is then manually shaked for 15 minutes and centrifuged for 10 minutes at 3000rpm. Following centrifugation, 3 layers are observed in the tube; name the layers accordingly and state what is contained within them:

. 13

Faq

Physics
Step-by-step answer
P Answered by Master

Answer:

see below.

Step-by-step explanation:

To solve this problem, we can use the conservation of energy and conservation of momentum principles.

Conservation of energy:

The total initial energy is the rest energy of the proton and neutron, which is given by:

Ei = (mp + mn)c^2

where mp and mn are the masses of the proton and neutron, respectively, and c is the speed of light.

The total final energy is the rest energy of the deuteron plus the energy of the gamma ray, which is given by:

Ef = (md)c^2 + Eg

where md is the mass of the deuteron and Eg is the energy of the gamma ray.

According to the conservation of energy principle, the initial energy and final energy must be equal, so we have:

Ei = Ef

(mp + mn)c^2 = (md)c^2 + Eg

Conservation of momentum:

The total initial momentum is zero because the proton and neutron are at rest. The total final momentum is the momentum of the deuteron and the momentum of the gamma ray. Since the gamma ray is massless, its momentum is given by:

pg = Eg/c

where pg is the momentum of the gamma ray.

According to the conservation of momentum principle, the total final momentum must be equal to zero, so we have:

0 = pd + pg

where pd is the momentum of the deuteron.

Solving for md and pd:

From the conservation of energy equation, we can solve for md:

md = (mp + mn - Eg/c^2)/c^2

Substituting this expression into the conservation of momentum equation, we get:

pd = -pg = -Eg/c

Substituting the given values, we have:

mp = 1.6726 × 10^-27 kg mn = 1.6749 × 10^-27 kg Eg = 2.2 × 10^6 eV = 3.52 × 10^-13 J

Using c = 2.998 × 10^8 m/s, we get:

md = (1.6726 × 10^-27 kg + 1.6749 × 10^-27 kg - 3.52 × 10^-13 J/(2.998 × 10^8 m/s)^2)/(2.998 × 10^8 m/s)^2 = 3.3435 × 10^-27 kg

pd = -Eg/c = -(3.52 × 10^-13 J)/(2.998 × 10^8 m/s) = -1.1723 × 10^-21 kg·m/s

Therefore, the mass of the deuteron is 3.3435 × 10^-27 kg, and its momentum is -1.1723 × 10^-21 kg·m/s.

Physics
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P Answered by PhD

The question specifies the diameter of the screw, therefore the IMA of this screw is 0.812? / 0.318 = 8.02

Physics
Step-by-step answer
P Answered by PhD
Answer:
7.25 secs.

Explanation:
First find the distance it takes to stop
s = [v^2-u^2]/2a = 0^2 - 8.7^2/2[-2.4] = 8.7^2/4.8
Next find the time it takes to go that distance , s = ut +[1/2] at^2
8.7^2/4.8 = 8.7t +[1/2] [ -2.4]t^2 , rearrange and
t^2 -[8.7/1.2]+ 8.7^2/[(1.2)(4.8)]=0 complete the square
[t - (8.7/2.4)]^2=0
t = 8.7/2.4 = 3.625 secs
At this stage the deceleration will push the object back in the direction it came from for another 3.625 secs when it will be 8.7 m/s again
Total time , T =2t = 7.25 secs.

Note:
The term differential is used in calculus to refer to an infinitesimal (infinitely small) change in some varying quantity. For example, if x is a variable, then a change in the value of x is often denoted Δx (pronounced delta x). The differential dx represents an infinitely small change in the variable x.
Physics
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P Answered by PhD
The change in temperature is 9.52°CExplanation:Since, the heat supplied by the electric kettle is totally used to increase the temperature of the water.Thus, from the law of conservation of energy can be stated as:Heat Supplied by Electric Kettle = Heat Absorbed by WaterHeat Supplied by Electric Kettle = m C ΔTwhere,Heat Supplied by Electric Kettle = 20,000 JMass of water = m = 0.5 kgSpecific Heat Capacity of Water = C = 4200 J/kg.°CChange in Temperature of Water = ΔTTherefore,20,000 J = (0.5 kg)(4200 J/kg.°C) ΔTΔT = 20,000 J/(2100 J/°C)ΔT = 9.52°C
Physics
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P Answered by PhD
Weight of barbell (m) = 100 kg
Uplifted to height (h) = 2m
Time taken= 1.5 s
Work done by Jordan = potential energy stored in barbell = mgh
= 100×2×9.8
= 1960J
Power = energy/time
= 1960/1.5
1306.67watts
Physics
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P Answered by PhD
Weight of jasmine (m) = 400 N
Height climbed on wall (h) = 5m
Total time taken in climbing = 5 sec
Work done in climbing the wall = rise in potential energy = mgh
= 400×9.8×51
= 19600J
Power generated by Jasmine = potential energy / time
= 19600/5
= 3920Watts
Physics
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P Answered by PhD
The horizontal and vertical motions of balloons are independent from each other.
Let vertical component of initial velocity U' horizontal component of initial velocity U"
Time of landing (t) is found with the help of vertical motion.
Since vertical component of initial velocity of balloon is zero(U' = 0)
From equation h = U't + 1/2gt^2
h = 1/2gt^2
t = √(2h/g)
t = √( 2×150/9.8)
t = 5.53 sec
Horizontal velocity = 50m/s
Horizontal range of balloon, R = U"t
= 50× 5.53
= 27.65m
So the balloon will go 27.65 metre away from the bridge
Physics
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P Answered by PhD
Gravity acceleration (g) = 9.8m/s^2
Time (t) = 3sec
Acceleration = velocity/time
Velocity = acceleration×time
= 9.8×3
= 29.4m/s
Physics
Step-by-step answer
P Answered by PhD
Initial velocity (u) = 0
Time taken = 4.5 seconds
Gravitational acceleration (g) = 9.8m/s^2
By the second equation of motion under gravity,
The distance that object fell down (h)
h = ut + (1/2)gt^2
h = 0×4.5 + (1/2)×9.8×(4.5)^2
h = 99.225 m
Physics
Step-by-step answer
P Answered by PhD
Gravitational acceleration (g) = 9.8m/s^2
Time of flight = 12 seconds
Acceleration = velocity/time
Velocity = acceleration × time
= 9.8×12
= 117.6 m/s

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