Biology : asked on sl1010
 01.01.2023

In actuality, the DNA sequence of the human genome is NOT random. Some sequences, including some very large sequences, are repeated many times throughout the human genome. Write two ideas you have for how this fact complicates the use of CRISPR gene-editing technology in humans.

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24.06.2023, solved by verified expert
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repeated DNA sequences decrease the target specificity of the CRISPR-Cas system, and may lead to off-target effects  

Explanation:

The CRISPR-Cas system is a versatile and powerful tool for gene editing. This gene-editing tool consists of a single guide RNA (sgRNA) that guides the Cas endonuclease protein to the appropriate genomic locus, where Cas generate a double-strand break in the DNA and thus induces DNA repair either by Non-homologous end joining (NHEJ) or Homologous Recombination (HR) repair pathways. The sgRNA consists of a 17-20 nucleotide sequence which is complementary to the target DNA, this sequence is called crispr RNA (crRNA). The existence of repeated DNA sequences hampers the specificity of the CRISPR-Cas tool because a particular sgRNA is able to guide Cas9 to different genomic loci that have the same (repeated) nucleotide sequence complementary to the crRNA. In consequence, repeated DNA sequences may lead to undesired off-target effects, i.e., unintended cleavage (and therefore unintended mutations) at untargeted genomic sites.

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Biology
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repeated DNA sequences decrease the target specificity of the CRISPR-Cas system, and may lead to off-target effects  

Explanation:

The CRISPR-Cas system is a versatile and powerful tool for gene editing. This gene-editing tool consists of a single guide RNA (sgRNA) that guides the Cas endonuclease protein to the appropriate genomic locus, where Cas generate a double-strand break in the DNA and thus induces DNA repair either by Non-homologous end joining (NHEJ) or Homologous Recombination (HR) repair pathways. The sgRNA consists of a 17-20 nucleotide sequence which is complementary to the target DNA, this sequence is called crispr RNA (crRNA). The existence of repeated DNA sequences hampers the specificity of the CRISPR-Cas tool because a particular sgRNA is able to guide Cas9 to different genomic loci that have the same (repeated) nucleotide sequence complementary to the crRNA. In consequence, repeated DNA sequences may lead to undesired off-target effects, i.e., unintended cleavage (and therefore unintended mutations) at untargeted genomic sites.

Biology
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Need this store of protein: 

The seed consists of three components: embryo, endosperm (sometimes perisperm), and seed-coat. Both endosperm and embryo are the products of double fertilization, whereas the seed-coat develops from the maternal, ovular tissues. The seed habit is a significant advancement in the evolution of higher plants.

Anatomy of seed :-

Need this store of protein: The seed consists of three components: embryo, endosperm (sometimesNeed this store of protein: The seed consists of three components: embryo, endosperm (sometimes

Seed storage proteins provide a source of amino acids and reduced N necessary for germination and early growth of the seedling.

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Astronomers are able to detect the dimmer star because its gravity causes the bright star to . Astronomers can also spot the dimmer star by observing a phenomenon called a(n) binary.

Astronomers are able to detect the dimmer star because its gravity causes the bright star to . AstroAstronomers are able to detect the dimmer star because its gravity causes the bright star to . Astro
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Fungi are a food source for animals and humans. Fungi produce oxygen to breathe. Fungi destroy rocks and minerals. Fungi are natural recyclers.

Fungi are a food source for animals and humans. Fungi produce oxygen to breathe. Fungi destroy rocks
Biology
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when soaked in dilute sodium chloride solution onion gain mass because of the process of osmosis.

 Explanation: Osmosis is a process when there is movement of water from the region of comparatively higher concentration to the region of lower concentration across in a permeable membrane.

Example with explaination :

when soaked in dilute sodium chloride solution onion gain mass because of the process of osmosis.&nb
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when soaked in dilute sodium chloride solution onion gain mass because of the process of osmosis. Explanation: Osmosis is a process when there is movement of water from the region of comparatively higher concentration to the region of lower concentration across in a permeable membrane.

when soaked in dilute sodium chloride solution onion gain mass because of the process of osmosis. Ex
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Answer: Tornadoes form when warm, humid air collides with cold, dry air. The denser cold air is pushed over the warm air, usually producing thunderstorms.

Biology
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the tiles to the correct boxes to complete the pairs.
Match the arteries and veins to their functions...

the tiles to the correct boxes to complete the pairs.Match the arteries and veins to their functionsthe tiles to the correct boxes to complete the pairs.Match the arteries and veins to their functions

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